workshops Archives

Advanced mushroom observation + identification

Description
With the summer mushroom season just around the corner, now is the perfect time to work on your observation and identification skills. This lecture builds on the Mushrooms 101 lecture from May and is aimed at anyone from ambitious beginners to the experienced. We will cover observation, photography, documentation, collection, identification and specimen preservation. We will also provide an introduction to the many useful and free resources that can be found online.

About Tom Bigelow
Tom Bigelow has been a member of the New York Mycological Society for 14 years and has served as the club’s president from 2016-21. He holds the record for iNat observations for the club, many of them rare. He’s renowned for his beautiful photographs of fungi, which have been chosen as iNat Observations of the Day on numerous occasions. He is an expert on polypores on crust fungi and has lectured to a number of clubs on the topic.

Sigrid Jakob

Instagram: @dung_fungi
iNat: @sigridjakob

About Sigrid Jakob
Sigrid Jakob is a dedicated recorder of the fungi of Green-Wood Cemetery and Prospect Park. She leads the conservation working group of the Fungal Diversity Survey (FunDiS) and does DNA sequencing of fungi at home. She has a particular interest in Russulas, Amanitas, the Incocybaceae and fungi growing on dung.

How to use microscopy measurement software

 

Elan Trybuch

Instagram: @elaniobro
iNat: @elaniobro
Twitter: @elaniobro

Description
Elan Trybuch researched how to measure spores in his spare time, where he discovered a plugin that was part of the Fiji open source ecosystem, that would do so. Elan forked the code on github and modified it to suit mycologists needs.

Learn how to use open source software Fiji to measure microscopic features and spores using the Microscope Measurement Tools plugin. You will also learn how to:

  • set different scales for each of your objectives on your microscope
  • add a scale bar and set the scale to your image
  • create macro shortcuts
  • use the measurement tool
  • customize the colors of the line, font, and text box

About Elan Trybuch
Elan Trybuch is a software engineer by trade and discovered his passion for fungi while out on a camping and rock climbing trip. He is the current secretary of the New York Mycological Society.

Mushroom DNA barcoding for the citizen scientist

Damon TigheChanges in DNA Sequencing technologies over the past decade have greatly reduced the cost of sequencing, making it available for non-academics and hobbyists. This presentation will cover the basic molecular biology involved in doing Sanger DNA Sequencing, the most commonly used technique for DNA Barcoding of Fungi. The tools and protocols involved will be covered at a few different levels, so that attendees can see how to get involved sequencing mushrooms of interest to them. Basic bioinformatics including BLAST, sequence aligners, and tree building tools will be stepped through with a focus on the freeware that I traditionally help high schoolers use. To see the power of adding DNA Sequencing to your identification toolbox a few example mushrooms will be covered.

About Damon Tighe:
Born in Klamath Falls Oregon and raised in Calaveras County of California, Damon Tighe attended Saint Mary’s college of Moraga California where he worked on local newspapers while earning a Biology/Chemistry degree. He taught High School in Portland, Oregon and moved back to the Bay Area to work on the Human Genome Project at the National Lab’s Joint Genome Institute. He spent a bit of time pursing a MFA in Natural History and Science Filmmaking in Montana, but returned to the Oakland to work on biofuels and single cell genomics. He currently manages an apartment complex in downtown Oakland, works for Bio-Rad laboratories designing curriculum and training educators from Colorado to the coast in biotechnology, and is well versed in local fungi and plants. The last few years he’s helped a number of high school classrooms start documenting their local fungi with iNaturalist and do DNA Barcoding.

Fungal microscopy

Alan RockefellerIn this lecture, you will learn why you might want to use a microscope, what types of microscope there are, how to choose a microscope, how to measure microscopic characteristics of fungi, and the kinds of chemicals and reagents that are useful in fungal microscopy. Alan will also cover the important microscopic characteristics of fungi, how to prepare your slides, how to take photographs of what you’re seeing through the microscopes. He will also go over different kinds of spores that can help with the identification of various genera of fungi.

About Alan Rockefeller:
Alan Rockefeller is a mushroom identification specialist, public speaker, biohacker, photographer, and consultant who lives in Oakland, California.

Alan began his autonomous studies in the field of mycology in 2001, beginning with the collective fungi in California. Since then, he has traveled to various countries to collect and study mushrooms. He has photographed and scoped well over 1000 species of fungi, with a particular focus on fungi in the genus Psilocybe as well as bioluminescent fungi, especially Mycena. Through phylogenetic and microscopic analysis, he has identified several species not described in the scientific literature and co-authored a number of papers on Psilocybe, Mycena and Amanita.

Alan regularly identifies mushrooms on websites such as Mushroom Observer, Facebook, the Shroomery and iNaturalist. In the lab Alan sequences the DNA of mushrooms from all over the world, gaining new insights into existing species, discovering new ones and eliminating duplicates in the taxonomic record. He lectures and teaches widely and his (recorded) lectures on DNA sequencing, microscopy and psilocybe have been a valuable resource to many. Alan is also chief mycologist at MIMOSA Therapeutics.

Mushrooms 101: An Introduction to Collecting and Identifying Fungi

Description
With the mushroom season just around the corner, now is the perfect time to learn how to identify common fungi of the Northeast. This lecture is aimed at beginners and will cover tools and terminology, the basics of identifying fungi, and how to differentiate the delicious from the deadly – whether you’re picking for the pot or simply like to put a name to the next mushroom you see. Bring questions for the Q&A!

About Ethan Crenson
Ethan Crenson received an MFA in photography from the School of Visual Arts in NYC in the 1990s. He runs two companies, a graphic design company and a gallery/publishing house for artists’ multiples. He became interested in fungi about 14 years ago and joined the New York Mycological Society shortly thereafter. He is an active contributor to the club’s five boroughs fungal survey, Gary Lincoff’s effort to inventory the fungal inhabitants of NYC. He has a keen interest in pyrenomycetous fungi and has presented programs on these “dots on sticks and scabs on branches” at NEMF and NAMA forays and on several occasions to the NYMS.

 

 

Sigrid Jakob

Instagram: @dung_fungi
iNat: @sigridjakob

About Sigrid Jakob
Sigrid Jakob is a dedicated recorder of the fungi of Green-Wood Cemetery and Prospect Park. She leads the conservation working group of the Fungal Diversity Survey (FunDiS) and does DNA sequencing of fungi at home. She has a particular interest in Russulas, Amanitas, the Incocybaceae and fungi growing on dung.